Fig. 1.

Sulphur treatment decreases IL-6 secretion in resting FLS. (A) FLS were treated with increasing concentrations of NaHS (0.06–1 mmol/l) for 1 hr and after 1 hr recovery in sulphur-free medium, IL-6 production was measured in cell culture supernatants by ELISA. (B) Cells were treated with PBS or 1 mmol/l NaHS for 1 hr following which they were allowed to recover in fresh medium for 3, 6 or 12 hrs. Supernatants were analysed for IL-6 concentration by ELISA. (C) Supernatants from (A) were further used to quantify human IL-8 and RANTES of FLS treated with 1 mmol/l NaHS or PBS. FLS from four different OA patients were used in these experiments. Each experiment was performed three times in duplicates or two times in triplicates (A) *P < 0.05; (B) *P < 0.05; (C) *P < 0.05, **P < 0.01.