Table 2.

Species richness and coverage estimation of 16S rRNA clone libraries generated from H. verbana microbial niches.

Richness estimation
Sample identity	Chao1	Chao2	ACE	ICE	Shannon-Weaver
Shed Mucus-3 days post shed	20.5	20.5	16.69	16.69	2.341
Gut-7 days post feedinga	11.25	11.25	11.1	11.1	1.112
Gut-90 days post feedinga	6	6	6	6	1.377
Bladderb	7	7	7	7	1.398

Isolates previously described in

^a(Worthen et al., 2006) richness estimation was calculated for combined crop and intestinum data;

^b(Kikuchi et al., 2009).