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. Author manuscript; available in PMC: 2015 Jan 9.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):2943–2952. doi: 10.1002/jcp.22639

Fig. 4.

Fig. 4

Effects of differential crosslinking of the hydrogels on OA release in the presence of mesenchymal cells, and release-dependent effects on mesenchymal cell differentiation. Gelatin hydrogels were constructed in 24-well tissue culture plates, loaded with 100 ng/well rmOA, and crosslinked with different concentrations of glutaraldehyde. A: OA release from hydrogels. Western blot analysis of media from C3H10T1/2 cells grown on rmOA-loaded hydrogels. Graph represents densitometric analysis of Western blot. rmOA release was time-dependent. B: ALP staining of C3H10T1/2 cells grown on rmOA-loaded hydrogels (molarities refer to glutaraldehyde concentration used to crosslink gelatin) at 14 days. rmOA release from the hydrogels had very little effect on cell differentiation as determined by ALP staining. 20× magnification.