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. Author manuscript; available in PMC: 2015 Jan 9.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):2943–2952. doi: 10.1002/jcp.22639

Fig. 5.

Fig. 5

Effects of differential loading of the hydrogels on mesenchymal cell differentiation. Hydrogels were constructed, loaded with rhBMP-2 at several loading doses (1, 2, or 4 μg/well) or with rmOA at several loading doses (1, 10, 50, or 100 ng/well), and crosslinked with 20 mM glutaraldehyde. A: Effects of differential loading of hydrogels with OA on ALP staining of C3H10T1/2 cells at 21 days. Concentrations represent loading concentrations of rmOA in gelatin solution. 20× magnification. B: Von Kossa staining of C3H10T1/2 cells grown on hydrogels differentially loaded with rmOA, and crosslinked with 20 mM glutaraldehyde. Concentrations represent loading concentrations of rmOA in gelatin solution. 20× magnification.