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. Author manuscript; available in PMC: 2015 Jan 9.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):2943–2952. doi: 10.1002/jcp.22639

Fig. 6.

Fig. 6

Concentrations of BMP-2 and OA administered to cells in bolus were titrated to give a maximum response of osteoblastic differentiation as determined by ALP staining in order to compare the osteoblastic effects of BMP-2 and OA. ALP staining of ROS 17/2 osteosarcoma cells grown in medium without growth factors demonstrated an increase in ALP staining with time in cultures. ALP staining of C3H10T1/2 cells at 7, 14, and 21 days: Cells shown in the OA group were grown in medium containing 100 ng/ml rmOA. Cells shown in the BMP-2 group were grown in medium containing 1 μg/ml rhBMP-2. In both conditions, there was an increase in ALP activity in response to rmOA and rhBMP-2, and this response was time-dependent.