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Forward (F) and reverse (R) primers for target genes
| Gene name | Amplicon length (bp) | 5′–3′ primer sequence |
|---|---|---|
| AKP-2 (NM007431) | 164 | F5′-TGCCTACTTGTGTGGCGTGAA-3′ |
| OC (NM007541) | 178 | R5′-TCACCCGAGTGGTAGTCACAATG-3′ F5′-AGCAGCTTGGCCCAGACCTA-3′ |
| β-Actin (NM007393) | 131 | R5′-TAGCGCCGGAGTCTGTTCACTAC-3′ F5′-TGACAGGATGCAGAAGGAGA-3′ |
| R5′-GCTGGAAGGTGGACAGTGAG-3′ |
