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. Author manuscript; available in PMC: 2015 Jan 10.
Published in final edited form as: Sci China Life Sci. 2014 Aug 16;57(9):867–875. doi: 10.1007/s11427-014-4730-2

Figure 5. Symptomatic relieve in a parkinsonian rat model mediated by hiDA cell derived from 5F+p53DN transduced IMR90 cells.

Figure 5

A. Amphetamine-induced rotational behaviors (duration:180 min) in 6-OHDA lesioned rats before cell engraftment, and at 4 and 8 weeks after the transplantation of reprogrammed cells (about 6×105, DIV12) or IMR90 cells (control) into the middle of striatum on the lesioned side. Transplantation of reprogrammed cells significantly prevented a steady increase in amphetamine-induced rotation scores in 6-OHDA lesioned rats at both 4 and 8 weeks after transplantation (*, p<0.05). Error bars indicate standard error of the mean (SEM, n=10 for reprogrammed cells group and n=4 for control group).

B. Coronal section of rat brain showing the 6-OHDA lesion area and the site of hiDA injection. Immunohistochemical analyses with tyrosine hydroxylase antibody and diaminobenzidine (TH/DAB) staining notices the strong staining of tyrosine hydroxylasein both the lesioned striatum and injection site.

C. Expression of tyrosine hydroxylase in reprogrammed cells at the injection site. TH; tyrosine hydroxylase; HN: human nucleus protein, a marker for injected, reprogramed human hiDA cells; Nucleus were stained with DAPI. The scale bar represents 50 μm.