Figure 4.

Relative weight change calculated as a percentage of the initial weight, in gamma-hydroxybutyrate-treated and control groups (black and gray bars, respectively). (A) Two-way repeated-measures analysis of variance for factors “treatment,” “week” and their interaction, followed by Holm-Sidak post hoc test showed that the weight gain by C57BL/6J mice in the treated group was significantly less compared to the control group; * P < 0.05, N = 20. Lines connect weeks for which significant differences were found. (B) The weight gain in obese (ob/ob) treated group was lower compared to the control group, but not significantly different between the treated and the control group (N = 16). (C) GABA_B KO treated mice lost weight compared with the control mice; * P < 0.05, N = 11. GABA_B, gamma-aminobutyric acid B; KO, knockout mice.