FIG 5.

Targeted Rot residues for site-directed mutagenesis. (A) Residues altered to generate mutant Rot proteins are shown in stick representation. Orange residues are predicted to make specific contacts with DNA. Purple residues are predicted to make nonspecific contacts with DNA. Red residues are predicted to be involved in interactions with protein partners. Distances between the Y⁶⁶ residues and the R⁹¹ residues in the two monomers are reported. (B) The WHTH region is shown in a closeup view with the side chains of selected residues in stick representation. The chloride ion is shown as a red sphere. (C and D) The HTH region is shown from two orientations. (E) Closeup view of the region predicted by ODA to be involved in interaction with protein partners.