FIG 6.

Mutant Rot proteins display functional defects. Mutant rot alleles carried on plasmid pOS1Plgt were transformed into an S. aureus reporter strain containing the sGFP gene under the control of the ssl7 promoter, which is activated by Rot. Left, Pssl7 activation by Rot containing group amino acid mutations in regions predicted to be important for Rot-mediated regulation. Right, Pssl7 activation by Rot proteins containing single amino acid mutations selected from group substitution mutants. Activation activity of Rot mutants was assessed by GFP fluorescence in cultures grown to post-exponential phase. Values are averages of results from three independent experiments ± standard deviations.