341 |
pssl7-F-PstI |
5′-CCCC-CTGCAG-GCAGACTAGTAATTGTAGGG |
Pssl7-sGFP reporter |
489 |
sGFP-R-BamHI |
5′-CCC-GGATCC-TTAGTGGTGGTGGTGGTGG |
Pssl7-sGFP reporter |
1118 |
Rot-Mut1-F-NdeI |
5′-CCCCCATATGATGAAAAAAGTAAATAACGACACTGTAGCAGGAATTGCAGCATTAGAAGCACTTTTGG |
Forward primer to make a Rot F10A/L13A/Q14A/T17A mutant; use with primer 311 to clone into pOS1Plgt
|
1120 |
Rot-Mut3-F |
5′-CAAAATGGCAAGAGCAGCAATTTTAATTTTACTAAC |
Forward SOEing primer to make a Rot S36A/E38A/E39A mutant; use with primer 311 |
1121 |
Rot-Mut3-R |
5′-GTTAGTAAAATTAAAATTGCTGCTCTTGCCATTTTG |
Reverse SOEing primer to make a Rot S36A/E38A/E39A mutant; use with primer 313 |
1122 |
Rot-Mut4-F |
5′-CTTTATGGGCAAAAGGTTCTATGAC |
Forward SOEing primer to make a Rot Q48A mutant; use with primer 311 |
1123 |
Rot-Mut4-R |
5′-GTCATAGAACCTTTTGCCCATAAAG |
Reverse SOEing primer to make a Rot Q48A mutant; use with primer 313 |
1124 |
Rot-Mut5-F |
5′-GGTTCTATGACGGCAGCAGAAATGGAC |
Forward SOEing primer to make a Rot L54A/K55A mutant; use with primer 311 |
1125 |
Rot-Mut5-R |
5′-GTCCATTTCTGCTGCCGTCATAGAACC |
Reverse SOEing primer to make a Rot L54A/K55A mutant; use with primer 313 |
1126 |
Rot-Mut6-F |
5′-GATTTGTTGAAGCAGCAGCAGCAGCAGCAGCAGCAACGTATAATAATTTAG |
Forward SOEing primer to make a Rot K64A/P65A/Y66A/K67A/R68A/T69A/R70A mutant; use with primer 311 |
1127 |
Rot-Mut6-R |
5′-CTAAATTATTATACGTTGCTGCTGCTGCTGCTGCTGCTGCTTCAACAAATC |
Reverse SOEing primer to make a Rot K64A/P65A/Y66A/K67A/R68A/T69A/R70A mutant; use with primer 313 |
1128 |
Rot-Mut7-F |
5′-CGAGAGCATATAATGCATTAGTTGAATTAG |
Forward SOEing primer to make a Rot T71A/N74A mutant; use with primer 311 |
1129 |
Rot-Mut7-R |
5′-CTAATTCAACTAATGCATTATATGCTCTCG |
Forward SOEing primer to make a Rot T71A/N74A mutant; use with primer 313 |
1132 |
Rot-Mut9-F |
5′-GACGATGAAGCAACAGTTATTATTC |
Forward SOEing primer to make a Rot R91A mutant; use with primer 311 |
1133 |
Rot-Mut9-R |
5′-GAATAATAACTGTTGCTTCATCGTC |
Reverse SOEing primer to make a Rot R91A mutant; use with primer 313 |
1137 |
Rot-Mut13-F |
5′-GAAGAAATTGCAATTTTAGCAACTTTATGGC |
Forward SOEing primer to make a Rot L41A/L44A mutant; use with primer 311 |
1138 |
Rot-Mut13-R |
5′-GCCATAAAGTTGCTAAAATTGCAATTTCTTC |
Reverse SOEing primer to make a Rot L41A/L44A mutant; use with primer 313 |
311 |
rot-6×His–3′-RXhoI |
5′-CCCCTCGAG-TTAGTGATGGTGATGGTGATG-CACAGCAATAATTGCGTTTAAAC |
Reverse primer to clone rot into pOS1-Plgt
|
313 |
rot5′F-NdeI |
5′-CCCC-CATATG-AAAAAAGTAAATAACGACACTG |
Forward primer to clone rot downstream of the lgt promoter in the pOS1-Plgt plasmid. |
270 |
hla P1-RT |
5′-AAAAAACTGCTAGTTATTAGAACGAAAGG |
qRT-PCR analysis of hla
|
271 |
hla P2-RT |
5′-GGCCAGGCTAAACCACTTTTG |
qRT-PCR analysis of hla
|
278 |
16S P1-RT |
5′-TGAGATGTTGGGTTAAGTCCCGCA |
qRT-PCR analysis of 16S rRNA |
279 |
16S P2-RT |
5′-CGGTTTCGCTGCCCTTTGTATTGT |
qRT-PCR analysis of 16S rRNA |
280 |
ssl7 P1-RT |
5′-AACGTTAGCTAAAGCAACATTGGC |
qRT-PCR analysis of ssl7
|
281 |
ssl7 P2-RT |
5′-TTGCTTGAACTGCTTGGCCTTCTG |
qRT-PCR analysis of ssl7
|
290 |
spA1P1-RT |
5′-CAGCAAACCATGCAGATGCTA |
qRT-PCR analysis of spa
|
291 |
spA2P2-RT |
5′-GCTAATGATAATCCACCAAATACAGTTG |
qRT-PCR analysis of spa
|
370 |
lukE-P1 |
5′-GAAATGGGGCGTTACTCAAA |
qRT-PCR analysis of lukE
|
371 |
lukE-P2 |
5′-GAATGGCCAAATCATTCGTT |
qRT-PCR analysis of lukE
|
305 |
pssl7-R-BIO |
5′-BIO-CCCC-AGTACTATTCTCCCAATCTATTT |
Biotinylated primer for EMSA probe amplification |
341 |
pssl7-F |
5′-CCCC-CTGCAG-GCAGACTAGTAATTGTAGGG |
EMSA probe amplification |
736 |
plukED-F-BIO |
5′-BIO-AAGTTTCACTTTCTTTCTATATAAAT |
Biotinylated primer for EMSA probe amplification |
397 |
plukED-R |
5′-CCC-CTGCAG-ATCTTCGTTTAACGGACAATAG |
EMSA probe amplification |