TABLE 4.
Plasmids used in this study
| Name | Description | Resistance | Reference |
|---|---|---|---|
| pOS1sGFP-Pssl7-sodRBS | ssl7 promoter and sod RBSa driving sGFP expression | Cm | 24 |
| pJC1111 | Suicide vector used for integration into the SaP-1 site | Cd | 57 |
| pJC1111 Pssl7-sodRBS-sGFP | ssl7 promoter and sod RBS driving sGFP expression cloned into the pJC1111 suicide vector | Cd | This study |
| pRN7023 | Vector encoding the SaP-1 integrase | Cm | 55 |
| pOS1Plgt | lgt promoter in an empty vector | Cm | 45 |
| pOS1Plgt rot | lgt promoter driving rot expression | Cm | 24 |
| pOS1Plgt rot F10A/L13A/Q14A/T17A | lgt promoter driving rot F10A/L13A/Q14A/T17A expression | Cm | This study |
| pOS1Plgt rot S36A/E38A/E39A | lgt promoter driving rot S36A/E38A/E39A expression | Cm | This study |
| pOS1Plgt rot Q48A | lgt promoter driving rot Q48A expression | Cm | This study |
| pOS1plgt rot L54A/K55A | lgt promoter driving rot L54A/K55A expression | Cm | This study |
| pOS1Plgt rot K64A/P65A/Y66A/K67A/R68A/T69A/R70A | lgt promoter driving rot K64A/P65A/Y66A/K67A/R68A/T69A/R70A expression | Cm | This study |
| pOS1Plgt rot T71A/N74A | lgt promoter driving rot T71A/N74A expression | Cm | This study |
| pOS1Plgt rot R91A | lgt promoter driving rot R91A expression | Cm | This study |
| pOS1Plgt rot L41A/L44A | lgt promoter driving rot L41A/L44A expression | Cm | This study |
| pET41b rot6xhis | rot expression vector | Kan | 31 |
a
RBS, ribosome binding site.