FIG 4.
Loss of relA leads to increased mobility on swim plates. (A) Quantitation of swim expansion from plate assays performed with wt (BJH001), ΔrelA (BQA009), and ΔrelA PrelA-relA (BQA068) strains on LB fortified with 0.25% agar. Each symbol indicates the average of measurements from five independent experiments with standard deviations. Measurements were recorded every 30 min for 8 h. (B) wt (BJH001), ΔrelA (BQA009), ΔrelA PrelA-relA (BQA068), ΔsigD (BQA022), Δhag (BQA076), ΔrelA ΔyjbM (BQA081), and ΔrelA ΔywaC (BQA082) strains were inoculated on LB fortified with 0.25% agar. After 3.5 h of incubation at 37°C, the plates were scanned against a black background. The images were inverted to better show contrast.