TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or descriptiona | Reference(s) or source |
|---|---|---|
| F. johnsoniae strains | ||
| UW101 (ATCC 17061) | Wild type | 27, 28 |
| CJ1631A | Δ(gldN gldO) in F. johnsoniae UW101 | 11 |
| CJ1808 | chiA disruption mutant; (Emr) | 7 |
| CJ1818 | porU disruption mutant; (Emr) | This study |
| CJ1827 | rpsL2; (Smr) “wild-type” strain for construction of all deletion mutants except CJ1631A | 29 |
| CJ1922 | rpsL2 ΔsprB; (Smr) | 29 |
| CJ1984 | rpsL2 ΔremA; (Smr) | 4 |
| CJ1985 | rpsL2 ΔsprB ΔremA; (Smr) | 4 |
| CJ2082 | rpsL2 ΔFjoh_0288; (Smr) | This study |
| CJ2083 | rpsL2 remA::myc tag 1; (Smr) | 4 |
| CJ2089 | rpsL2 Δ(gldN gldO) remA::myc tag 1; (Smr) | 4 |
| CJ2090 | rpsL2 Δ(gldN gldO); (Smr) | 4 |
| CJ2116 | rpsL2 ΔporU; (Smr) | This study |
| CJ2130 | rpsL2 ΔporV; (Smr) | This study |
| CJ2323 | rpsL2 ΔporV remA::myc tag 1; (Smr) | This study |
| CJ2445 | rpsL2 ΔporV ΔsprB; (Smr) | This study |
| CJ2446 | rpsL2 ΔporV ΔFjoh_0288; (Smr) | This study |
| Plasmids | ||
| pCP23 | E. coli-F. johnsoniae shuttle plasmid; Apr (Tcr) | 31 |
| pCP29 | E. coli-F. johnsoniae shuttle plasmid; Apr (Cfr Emr) | 45 |
| pRR51 | rpsL-containing suicide vector; Apr (Emr) | 29 |
| pRR39 | pCP23 carrying remA; Apr (Tcr) | 4 |
| pSSK01 | 1,050-bp fragment of porU in pLYL03 for constructing porU disruption mutant CJ1818; Apr (Emr) | This study |
| pSSK03 | 1,516-bp SphI-KpnI fragment spanning porV amplified with primers 972 and 973 and inserted into pCP29; Apr (Cfr Emr) | This study |
| pSSK04 | 4,309-bp XbaI-BamHI fragment spanning porU amplified with primers 988 and 989 and inserted into pCP23; Apr (Tcr) | This study |
| pSSK14 | 2,332-bp XbaI-SalI fragment downstream of Fjoh_0288 amplified with primers 1104 and 1105 and inserted into pSSK16; Apr (Emr) | This study |
| pSSK16 | 2,301-bp BamHI-XbaI fragment upstream of Fjoh_0288 amplified with primers 1102 and 1103 and inserted into pRR51; Apr (Emr) | This study |
| pSSK20 | 2,442-bp BamHI-SalI region upstream of porV amplified with primers 1203 and 1204 and inserted into pRR51; Apr (Emr) | This study |
| pSSK21 | 2,282-bp BamHI-SalI region upstream of porU amplified with primers 1207 and 1208 and inserted into pRR51; Apr (Emr) | This study |
| pSSK22 | 2,271-bp SalI-SphI region downstream of porV amplified with primers 1201 and 1202 and inserted into pSSK20; Apr (Emr) | This study |
| pSSK23 | 2,305-bp SalI-SphI region downstream of porU amplified with primers 1205 and 1206 and inserted into pSSK21; Apr (Emr) | This study |
| pTB79 | pCP23 carrying gldN; Apr (Tcr) | 46 |
a
Antibiotic resistance phenotypes are as follows: ampicillin, Apr; cefoxitin, Cfr; erythromycin, Emr; streptomycin, Smr; tetracycline, Tcr. The antibiotic resistance phenotypes given in parentheses are those expressed in F. johnsoniae but not in E. coli. The antibiotic resistance phenotypes without parentheses are those expressed in E. coli but not in F. johnsoniae.