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Expression of flagellin (FlaA) in wild-type and ΔfliL mutant cells. Shown are transcription (A) and translation (B) of flaA, measured as β-galactosidase activity from a PflaA::lacZ transcriptional fusion and flaA′::′lacZ translational fusion, respectively, in wild-type (solid circles) and ΔfliL (open circles) cells. Means ± standard deviations are shown (n = 3). (C) Immunoblot to flagellin in wild-type (W) and ΔfliL (L) cells.
