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Cell morphology and flagellin expression of wild-type and ΔfliL mutant cells at 25°C. (A) Phase-contrast micrographs (400× magnification) of ΔfliL cells obtained from LB broth after 6 h or hard LB agar at 8 h of growth at 25°C. Scale bar, 10 μm. (B) Immunoblot to flagellin in the wild-type (WT) and ΔfliL cells grown in LB broth for 6 h or LB agar for 8 h.
