TABLE 2.
COMSTAT analysis of PAO1, PAO1 ΔgcbA, PAO1/pJN105, and PAO1/pJN-gcbA biofilmsa
| Strain | Total biomass (μm3/μm2) | Substratum coverage (%) | Avg thickness (μm) | Roughness coefficient | Maximum thickness (μm) |
|---|---|---|---|---|---|
| PAO1 | 7.27 (±1.85) | 22.27 (±6.90) | 7.61 (±2.15) | 1.41 (±0.15) | 42.13 (±8.93) |
| PAO1 ΔgcbA | 9.28 (±3.17) | 40.88 (±16.36)* | 9.82 (±3.46) | 1.02 (±0.24) | 45.33 (±9.19) |
| PAO1/pJN105 | 6.16 (±2.85) | 14.08 (±4.15) | 6.22 (±3.04) | 1.68 (±0.09) | 48.43 (±9.30) |
| PAO1/pJN-gcbA | 3.78 (±3.29)* | 8.18 (±5.84)* | 3.95 (±3.59)* | 1.81 (±0.14) | 45.43 (±14.24) |
a
COMSTAT analysis was carried out on 144-h-old biofilms grown in triplicate, using at least 6 images per replicate. Values are means (±standard deviation). *, significantly different from the results for wild-type biofilms (P ≤ 0.01).