FIG 2.

Confirmation of strains Δchp2 and ΔmmpL10 by PCR. For chp2, the 5′ primer annealed ∼900 bp upstream of chp2. For lanes 1 and 3, the 3′ primer annealed to a region of chp2 that is absent in the knockout strain. For mmpL10, the 5′ primer annealed ∼100 bp upstream of mmpL10. For lanes 5 and 7, the 3′ primer annealed to a region of mmpL10 that is absent in the knockout strain. For lanes 2, 4, 6, and 8, the 3′ primer annealed to the hygR marker that replaces chp2 and mmpL10 in the knockout strains. See Table S2 in the supplemental material for oligonucleotide sequences.