FIG 4.
LarR multimerization. (A) DNA sequence of the central part of the L. plantarum larR-larA intergenic region. The putative −35 and −10 boxes of PlarR and PlarA, the Lar box, and the mapped transcriptional start (+1) of larA are highlighted in black. Protected regions in footprinting assays (lines) and sites displaying enhanced sensitivity to DNase I upon binding of LarR (V), such as are displayed in Fig. 6C, are indicated above the sequence. The half-Lar boxes surrounding the central Lar box are shown by arrows above the sequence. (B) Comparison of the half-Lar box sequences with the consensus Lar box sequence. (C) EMSA of a 256-bp 32P-radiolabeled probe of the larR-larA intergenic region of L. plantarum in the presence of increasing amounts of purified rLarR. The lower-molecular-weight rLarR-DNA complex (C1), unbound probe (P), and high-molecular-weight complexes (HMWC) are indicated.