FIG 3.

LPS phenotypes conferred by different Wzy_Sf mutants expressed in PNRM6 [Δwzy_Sf(pAC/pBADT7-1)]. Plasmid-encoded mutated Wzy_Sf proteins were expressed in PNRM6. The strains were grown and induced with arabinose as described in Materials and Methods. LPS samples were prepared, electrophoresed on SDS–15% (wt/vol) PAGE gels, and silver stained (see Materials and Methods). The strains were divided into various mutant classes (A, B, C, and D) based on their LPS phenotypes. Lane 1, positive-control strain PNRM13 [PNRM6(pRMPN1)]; lanes 2 to 10, Δwzy strain (PNRM6) with plasmids encoding mutated Wzy_Sf proteins, as indicated. The position of R-LPS is indicated. The numbers on the left indicate the Oag RUs. WT, wild type.