FIG 4.

Comparison of the LPS phenotypes conferred by the Wzy_Sf mutants expressed in the Δwzy and Δwzy Δwzz backgrounds. Plasmids encoding mutated Wzy_Sf proteins were expressed in PNRM126 [RMA4337(pAC/pBADT7-1)] and PNRM6 [RMM109(pAC/pBADT7-1)]. The strains were grown and induced as described in Materials and Methods. LPS samples were electrophoresed on SDS–15% (wt/vol) PAGE gels and silver stained (see Materials and Methods). Lanes 1 to 3 contain the indicated strains; lanes 4 to 21 contain the Δwzy (PNRM6) or Δwzy Δwzz (PNRM126) strain with plasmids encoding the indicated mutated Wzy_Sf proteins.