FIG 4.

BetI regulates quorum-sensing genes. The transcript levels of Qrr1 to Qrr5 in V. harveyi strains induced with 1 mM IPTG were assayed by qRT-PCR. The strains tested were a luxO D47E strain carrying a control vector (white bars; JAF548::pJV298) and a luxO D47E strain carrying a vector expressing betI from an IPTG-inducible promoter (black bars; JAF548::pJV299). Error bars represent the standard errors of measurement from three biological replicates, and these data represent those from two independent experiments.