FIG 5.

Importance of Arg75 of YidC2 for its growth-supporting function. (A) Schematic representations of the strains used for growth complementation assays. They have the chromosomal spoIIIJ deleted and have plasmids that carry spoIIIJ under the control of the IPTG-inducible Pspac promoter. Their yidC2 genotypes are indicated by yidC2*, representing either the wild type, deletion, or R75A at the native locus on the chromosome. (B) Growth complementation. Serially diluted cultures of B. subtilis strains SCB3045 (ΔyidC2), SCB3722 (yidC2⁺), and SCB3723 [yidC2(R75A)] were spotted onto LB agar plates with or without IPTG and incubated at 37°C for 17.5 h.