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. 2015 Jan 8;9(1):e3404. doi: 10.1371/journal.pntd.0003404

Figure 5. Bayesian phylogeny of Trypanozoon isolates based on the dihydrolipoamide dehydrogenase gene (LipDH; Tb927.11.16730).

Figure 5

Panel A shows a mid-point rooted tree based on an alignment of 32 unique LipDH haplotypes, assembled from sequences derived from 13 T. b. brucei (Tbb), 3 T. b. gambiense type 1 (Tbg1), 4 T. b. rhodesiense (Tbr), 15 T. evansi (Tev) and 5 T. equiperdum (Teq) samples. Scale units for the phylogeny are substitutions per site. The chart illustrates the distribution of each haplotype among samples from each Trypanozoon taxon. Phylogenetic analysis grouped all but three of the haplotype sequences into one of five major clusters with strong support (posterior probabilities ≥0.9), which are referred to as clades V, W, X, Y and Z. Eight unique Tev/Teq genotypes were found, as summarized in panel B. Discounting minor sequence differences (1–2 SNPs) these were reduced to four major genotypes based on the position of haplotypes in the tree, which mirrored the type of mutation (A281Δ, M282L, A273P, WT) in the C-termini of the ATP synthase subunit γ in these the samples.