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. Author manuscript; available in PMC: 2015 Jan 10.
Published in final edited form as: FASEB J. 2006 Feb 2;20(6):705–707. doi: 10.1096/fj.05-4661fje

Figure 5.

Figure 5

Caveolin-3 moves away from the sarcolemma and interacts with GLUT4 after insulin stimulation. A) Skeletal muscle cells were differentiated for 5 days. Cells were left untreated (left panel) and treated with insulin (160 nM) for 5 (middle panel) and 10 (right panel) min. Cells were then incubated with antibody probes specific for caveolin-3 and GLUT4, followed by incubations with fluorescent secondary antibodies. Green: caveolin-3; red: GLUT4; yellow indicates colocalization between caveolin-3 and GLUT4. B) Differentiated myotubes were left untreated or treated with 160 nM insulin for different periods of time (2, 5, and 20 min). Cell lysates were immunoprecipitated with anti caveolin-3 IgGs. Immunoprecipitates were then subjected to immunoblot analysis with an antibody probe specific for flotillin-1. Insulin did not change caveolin-3 and flotillin-1 total protein expression (lower panels).