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. Author manuscript; available in PMC: 2015 Jan 10.
Published in final edited form as: J Pharmacogenomics Pharmacoproteomics. 2014 Sep 30;5(4):1000141. doi: 10.4172/2153-0645.1000141

Figure 1. RSV and hMPV infection modulates ARE-dependent gene transcription.

Figure 1

(A) A549 cells were transiently transfected with a plasmid containing multiple copies of the NQO1 ARE site linked to the luciferase gene and then infected with either RSV (Left panel) or hMPV (Right panel). Cells were harvested at different times post-infection to measure luciferase activity. Uninfected cells, transfected with reporter plasmid only and mock-infected, served as controls. For each plate luciferase was normalized to the β-galactosidase reporter activity. Data are expressed as mean ± standard deviation of normalized luciferase activity. *P <0.05 relative to RSV or hMPV infected cells. (B) Nuclear extracts prepared from A549 cells infected with RSV (left panel) or hMPV (right panel) for various periods of time post infection (p.i.) were subjected to western blot with anti Nrf2 antibody. Membranes were stripped and reprobed for lamin B as an internal control for protein integrity and loading.