FIG 1.

Antigen-specific IL-17A and IFN-γ responses elicited by rSaEsxA or rSaEsxB immunization. Immunized mice (rSaEsxA or rSaEsxB plus AHG) (n = 8) were sacrificed 5 days after the third immunization, and splenocytes were prepared and stimulated with rSaEsxA or rSaEsxB protein for 20 h. IL-17A (A)- and IFN-γ (B)-producing cells were detected by ELISPOT assay. (C) Representative images of splenic ELISPOT responses. Results of one of two representative experiments are shown. Student's t test was used for the statistical analysis. Data are expressed as means ± standard errors of the means (SEM). SFU, spot-forming units; IMSA, immunized mice stimulated with SaEsxA; IMSB, immunized mice stimulated with SaEsxB; NMSA, naive mice stimulated with SaEsxA; NMSB, naive mice stimulated with SaEsxB; UNS, unstimulated immunized mice.