TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype/descriptiona | Reference |
|---|---|---|
| Strains | ||
| S. Typhimurium JSG210 | ATCC 14028s (WT) | 12 |
| S. Typhimurium ALZ43 | JSG210 Δhyb::FRT Δhyd::FRT Δhya::FRT (triple mutant) | 12 |
| S. Typhimurium RLK5 | JSG210ΔhycB::FRT (ΔhycB) | This study |
| S. Typhimurium RLK7 | JSG210ΔhycC::FRT (ΔhycC) | This study |
| S. Typhimurium RLK9 | ALZ43ΔhycC::FRT (quadruple mutant) | This study |
| Plasmids | ||
| pCP20 | Contains flippase gene for λ Red mutagenesis; Ampr | 30 |
| pKD46 | Contains λ Red genes γ, β, and exo; Ampr | 30 |
| pKD4 | Contains aphA3 cassette; Kanr | 30 |
a
Amp, ampicillin; FRT, flippase recombinase recognition target; Kan, kanamycin; WT, wild type.