TABLE 2.

Primers used in this study

Primer	Primer sequence (5′ → 3′)	Application
hycB del-F	CAAAAATGACAATCACCTGAGGAATGCCTGTGTGTAGGCTGGAGCTGCTTC	hycB deletion
hycB del-R	GACGCCGCTAGTAATCAGTGAAAGTGAACTCATATGAATATCCTCCTTA	hycB deletion
hycB-check-F	ACGTTGAAACCAAAGATGGCGA	hycB deletion confirmation
hycB-check-R	AATTTGCAGCATGTGGCCGGTAAA	hycB deletion confirmation
hycC del-F	CTTGTTTCAGCAGGCTCAGAGTGGGGATGCATATGTGTAGGCTGGAGCTGCTTC	hycC deletion
hycC del-R	GCGCCTGAATTAACGGATAAAACACACTCATTTCATATGAATATCCTCCTTA	hycC deletion
hycC-check-F	GTGAGCTGACGTTTAATACCGA	hycC deletion confirmation
hycC-check-R	CGACCGAGCAGTTTGATAATGT	hycC deletion confirmation