TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| E. faecalis strains | ||
| V19 | Strain V583 (clinical isolate), without plasmid | 14 |
| V19 ΔslyA | V19 isogenic derivative; ΔslyA mutant | 9 |
| V19/p3535-pmvE | Strain V19 harboring plasmid pMSP3535-pmvE | This study |
| V19/p3535 | Strain V19 harboring plasmid pMSP3535 | This study |
| E. coli strains | ||
| XL1Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIq ZΔM15] Tn10 (Tetr) | Stratagene |
| M15(pREP4) | pQE30 host strain | Qiagen |
| Plasmids | ||
| pQE30 | Ampr; expression vector | Qiagen |
| pMSP3535 | Ermr nisR nisK PnisA (nisin-inducible promoter) | 16 |
| pMSP3535-pmvE | pmvE cloned into overexpression vector pMSP3535 | This study |