TABLE 2.
Gold particle distribution in Leishmania major promastigotes under control conditions and treated with H2O2a
| Location | No. of gold particles (mean ± SD) under indicated condition |
||
|---|---|---|---|
| Control | Treatment with 10 μM H2O2 | Treatment with 25 μM H2O2 | |
| Nucleus | 9.25 ± 1.90 | 4.25 ± 1.85 | 1.57 ± 0.20 |
| Kinetoplast | 3.66 ± 0.90 | 3.25 ± 1.04 | 3.00 ± 0.35 |
| Mitochondrion | 3.00 ± 0.50 | 4.77 ± 0.46 | 1.79 ± 0.27 |
| Flagellum | 3.05 ± 1.25 | 3.06 ± 1.02 | 2.19 ± 0.30 |
| Membrane | 2.33 ± 0.30 | 3.33 ± 0.55 | 4.05 ± 1.20 |
| V/FP/C | 1.25 ± 0.75 | 1.75 ± 0.47 | 3.21 ± 0.32 |
| Nanovesicles | 1.50 ± 0.60 | 4.50 ± 0.90 | 7.11 ± 0.70 |
a
Cell slices were first incubated with anti-TEL-1 antibodies and then with anti-rabbit antibodies labeled with 25-nm gold particles. Sixty-five cells were examined for each condition. V, vesicles; FP, flagellar pocket; C, cytoplasm.