FIG 4.

V. cholerae response to TC during detachment. (A) Level of expression of vpsA in detached cells, as measured with the β-galactosidase assay. Cells were collected following 2 h of detachment. TC and DC were supplemented to a final concentration of 1 mM. (B) Following growth for 24 h in biofilm-inducing conditions, wild-type V. cholerae biofilms were incubated with the protein synthesis inhibitor chloramphenicol (Cm) and TC for 2 h. Remaining biofilm was quantified with CV staining. Data are means and SD from three independent experiments. NS, no significance; **, P < 0.005; ****, P < 0.0001.