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. Author manuscript; available in PMC: 2015 May 25.
Published in final edited form as: Nat Commun. 2014 Nov 25;5:5601. doi: 10.1038/ncomms6601

Figure 1. Phosphorylation of MeCP2 S421 is regulated by cell cycle in aNPCs.

Figure 1

(a,b) Western blot analysis of MeCP2 S421 phosphorylation and the quantification of total MeCP2 protein level in WT and phosphor-mutant aNPCs. (p=0.718, unpaired t-test with Welch’s correction, n=3 in each group) (c,d) Western blot analysis and quantification of the relative MeCP2 S421 phosphorylation level in WT aNPCs under normal proliferating condition and FGF2/EGF withdrawal for 24 hours. (n=3 in each group) (e) Western blot analysis reveals that MeCP2 S421 phosphorylation is induced by synchronizing aNPCs with nocodazole (150ng/ml). (f,g) Western blot analysis and quantification of the relative MeCP2 S421 phosphorylation level in aNPCs under conditions: 1) DMSO, 2) 36 hours of nocodazole treatment, 3) 24 hours of roscovitine (25 μM) treatment after pre-synchronization of the cells by nocodazole for 12 hours. (n=3 in each group) Numbers next to Western blots are molecular weight markers. The bar graph shows the mean ± s.e.m * p<0.05 ** p<0.01