Table 1.
Binding studies of 18-crown-6 and PEG400 to Pin1R14A, DMP19, and RbmA.a
| Additive | No additive | 18-Crown-6 | PEG400 | ||||
|---|---|---|---|---|---|---|---|
| [mmol L−1] | 0 | 10 | 50 | 100 | 10 | 50 | 100 |
| Protein | |||||||
| Pin1R14A | 50±0 | 49.5±0 | 49±0 | 49±0 | 48±0 | 42.5±0 | 30.8±0.8 |
| DMP19 | 53.8±0.3 45.3±0.8 | 50.7±0.6 46.3±1 | 48.8±0.3 28.7±2.9 | 45.0±0 25.3±0.6 | 53.8±0.3 44.8±0.3 | 53.6±0.3 45.3±0.3 | 53.3±0.3 45.8±0.3 |
| RbmA | 46±0 | 44.6±0.3 | 41.3±0.3 | 38.0±0 | 46.2±0.3 | 46.5±0 | 46.8±0.3 |
a
Protein–additive mixtures were analyzed by thermofluorassay with variable concentrations of additives. Fluorescence intensities (FI) were measured at increasing temperatures T. The table shows only the melting temperatures from all assays, with values in °C. Melting curves are provided in the Supporting Information, Figure S1.