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. Author manuscript; available in PMC: 2015 Jan 10.
Published in final edited form as: Mol Cancer Ther. 2013 Sep 12;12(11):2483–2493. doi: 10.1158/1535-7163.MCT-13-0486

Figure 5.

Figure 5

Effects of WIN and Sp protein knockdown on total and phospho-eIF4E in SW480 cells. (A) Cells were treated with DMSO or 7.5 μM WIN for 12, 18, 24, 36 and 48 hr, and whole cell lysates were analyzed by western blots, and levels of expression relative to DMSO (set at 1.0) are indicated. Cells were pretreated with SOV for 45 min (B) or transfected with siRNA against PP2A (C), followed by treatment with WIN for 48 hr, and whole cell lysates were analyzed by western blots. (D) SW480 cells were transfected with various siRNA and whole cell lysates were analyzed for expression of Sp proteins and eIF4E by western blots. iLamin was used as a control oligonucleotide.