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. Author manuscript; available in PMC: 2015 Jan 10.
Published in final edited form as: Mol Cancer Ther. 2013 Sep 12;12(11):2483–2493. doi: 10.1158/1535-7163.MCT-13-0486

Figure 6.

Figure 6

WIN modulates miR27a and ZBTB10 expression. (A) SW480 cells were treated with DMSO or 7.5 μM of WIN for 12, 18, 24, 36 and 48 hr, and whole cell lysates were analyzed for ZBTB10 protein by western blots. SW480 cells were transfected with siRNA against PP2A, followed by treatment with 7.5 μM WIN for 24 and 48 hr. Whole cell lysates were analyzed by western blot analysis for ZBTB10 expression (B) and miR27a levels (C) were determined by qPCR as described in the Materials and Methods. (D) SW480 cells were transfected with siRNA against ZBTB10 followed by treatment with 7.5 μM WIN for 24 and 48 hr, and whole cell lysates were analyzed by western blots. Results for RNA expression are expressed as means ± SE for at least three separate determinations, and significant (p < 0.05) inhibition (*) by WIN is indicated and reversal of these effects by iPP2A (**) are indicated.