Fig. 6.

Protein levels of TGF-β1 and α-SMA in lung tissues

The protein levels of TGF-β1 and α-SMA were significantly reduced by RAPA treatment for 14 d (a–c) and 28 d (d–f), respectively. Data are expressed as mean±SEM (n=6). ^* P<0.05, ^** P<0.01, ^*** P<0.001, compared with control; ^# P<0.05, ^## P<0.01, ^### P<0.001, compared with PQ group; ^†† P<0.01, ^††† P<0.001, compared with PQ+MP group. The blots with anti-TGF-β1 antibody and anti-α-SMA antibody are shown in (a) and (d), respectively, and densitometric analysis normalized against β-actin is shown in (b), (c), (e), and (f)