Extended Data Table 1.

Data collection, phasing and refinement statistics

	RNF146(RING-WWE)/UbcH5a/iso-ADPr complex Zn2+ SAD
Data collection
Space group	P21212
Cell dimensions
a, b, c (Å)	133.67,61.69,94.35
α, β, γ(°)	90, 90, 90
Resolution (Å)	50.0-1.90(1.96-1.90)*
Rsym(%)	9.2 (45.9)*
I/σI	34.4 (2.5)*
Completeness (%)	95.6(71.4)*
Redundancy	7.8 (4.6)*
Refinement
Resolution (Å)	50.0-1.90
No. reflections (test set)	55779(2994)
Rwork/Rfree	18.6/22.2
No. atoms
Protein	4886
Ligand	72
Zn2+	4
Water	371
B-factors
Protein	16.3
Ligand	31.0
Zn2+	31.5
Water	42.4
R.m.s deviations
Bond lengths (Å)	0.009
Bond angles (°)	1.4

This diffraction dataset was collected from a single crystal. 5% randomly selected reflections were used as a test set.

^*Highest resolution shell is shown in parenthesis.