Table 1.
Proteins identified as differentially-expressed following exposure to nitrosative stress
| Uniprot access | Proteins | Score | Peptides | log 2 DETA:CT (a) | p -value (a) | Subcellular localization (c) | Gene name | Genome (b) |
|---|---|---|---|---|---|---|---|---|
| Transport | ||||||||
| F9Y2Z3_CORP1 | Cell wall channel | 5321.88 | 4 | 1.42 | 1 | CYT | porH | Shared |
| Cell division | ||||||||
| D9Q7G2_CORP1 | Hypothetical protein | 2417.8 | 21 | 1.34 | 1 | CYT | Cp1002_0716 | Core |
| DNA synthesis and repair | ||||||||
| D9Q5V6_CORP1 | Nucleoid-associated protein | 2327.08 | 5 | 1.52 | 1 | CYT | ybaB | Core |
| D9Q923_CORP1 | Methylated-DNA-protein-cysteine methyltransferase | 6332.83 | 8 | 1.22 | 1 | CYT | ada | Core |
| D9Q4P0_CORP1 | 7,8-dihydro-8-oxoguanine-triphosphatase | 1640.23 | 8 | -1.97 | 0 | CYT | mutT | Core |
| Transcription | ||||||||
| D9Q8W2_CORP1 | LexA repressor | 800.31 | 6 | -1.37 | 0.04 | CYT | lexA | Shared |
| D9Q5L4_CORP1 | ECF family sigma factor k | 364.82 | 8 | -1.58 | 0 | CYT | sigK | Core |
| Translation | ||||||||
| D9Q753_CORP1 | Fkbp-type peptidyl-prolyl cis-trans isomerase | 7113.34 | 3 | 2.43 | 1 | CYT | fkbP | Core |
| D9Q830_CORP1 | 50S ribosomal protein L35 | 2271.66 | 1 | 1.36 | 1 | CYT | rpmI | Core |
| D9Q7W1_CORP1 | Aspartyl glutamyl-tRNA amidotransferase subunit C | 3100.8 | 7 | 1.24 | 0.99 | CYT | gatC | Core |
| D9Q582_CORP1 | 50S ribosomal protein L9 | 41082.46 | 10 | -1.25 | 0 | CYT | rplI | |
| D9Q6H6_CORP1 | 30S ribosomal protein S8 | 45333.23 | 9 | -1.34 | 0 | CYT | rpsH | Core |
| Cell communication | ||||||||
| D9Q559_CORP1 | Hypothetical protein | 1402.27 | 6 | 1.99 | 1 | PSE | Cp1002_2005 | Core |
| D9Q5U9_CORP1 | Thermosensitive gluconokinase | 2068.35 | 7 | 1.96 | 0.99 | CYT | gntK | Core |
| D9Q668_CORP1 | Sensory transduction protein RegX3 | 2540.92 | 13 | 1.45 | 1 | CYT | regX3 | Core |
| Detoxification | ||||||||
| D9Q7U6_CORP1 | Thioredoxin | 1835.7 | 11 | 1.50 | 1 | CYT | trxA | Core |
| D9Q4E5_CORP1 | Glutathione peroxidase | 1426.27 | 10 | 1.47 | 1 | CYT | Cp1002_1731 | Core |
| D9Q5T5_CORP1 | Glyoxalase bleomycin resistance protein dihydroxybiphenyl dioxygenase | 2417.77 | 11 | 1.28 | 1 | CYT | Cp1002_0124 | Shared |
| D9Q5N2_CORP1 | NADH dehydrogenase | 7030.94 | 12 | 1.25 | 1 | CYT | noxC | Shared |
| D9Q680_CORP1 | Glutaredoxin-like domain protein | 292.69 | 2 | -1.91 | 0 | CYT | Cp1002_0272 | Core |
| Glycolysis pathways | ||||||||
| D9Q5B6_CORP1 | N-Acetylglucosamine kinase | 228.69 | 6 | 1.74 | 0.98 | CYT | nanK | Core |
| D9Q4U9_CORP1 | Alcohol dehydrogenase | 236.02 | 17 | 1.22 | 1 | CYT | adhA | Shared |
| Iron-sulfur clusters | ||||||||
| D9Q7L6_CORP1 | Ferredoxin | 36927.57 | 7 | 2.10 | 1 | CYT | fdxA | Core |
| Antibiotic resistance | ||||||||
| D9Q827_CORP1 | Metallo-beta-lactamase superfamily protein | 657.33 | 6 | -2.95 | 0 | CYT | Cp1002_0937 | Core |
| Amino acid metabolism | ||||||||
| D9Q622_CORP1 | Phosphoserine phosphatase | 949.15 | 9 | 1.58 | 0.99 | PSE | serB | Core |
| D9Q4N1_CORP1 | Carboxylate-amine ligase | 205.54 | 16 | 1.24 | 1 | CYT | Cp1002_1819 | Core |
| D9Q6H4_CORP1 | L-serine dehydratase I | 284.11 | 17 | -1.37 | 0 | MEM | sdaA | Core |
| Lipid metabolism | ||||||||
| D9Q520_CORP1 | Glycerophosphoryl diester phosphodiesterase | 2417.8 | 21 | 1.34 | 1 | PSE | glpQ | Core |
| Oxidative phosphorylation | ||||||||
| D9Q8I5_CORP1 | Cytochrome aa3 controlling protein | 676.2 | 6 | 1.28 | 1 | MEM | Cp1002_1095 | Core |
| Specific metabolic pathways | ||||||||
| D9Q5M9_CORP1 | Inositol-3-phosphate synthase | 7473.38 | 18 | 2.25 | 1 | CYT | ino1 | Core |
| D9Q721_CORP1 | Hypothetical protein | 4602.9 | 17 | 1.44 | 1 | SEC | Cp1002_0573 | Core |
| D9Q689_CORP1 | 3-Hydroxyisobutyrate dehydrogenase | 2137.24 | 12 | 1.34 | 1 | CYT | mmsB | Core |
| D9Q4X1_CORP1 | Urease accessory protein UreG | 1532.39 | 12 | -1.6 | 0 | CYT | ureG | Core |
| Nucleotide metabolism | ||||||||
| D9Q4S2_CORP1 | Orotate phosphoribosyltransferase | 2618.52 | 8 | -1.26 | 0 | CYT | pyrE | Core |
| Unknown function | ||||||||
| D9Q6Y9_CORP1 | Hypothetical protein | 491.89 | 10 | 2.87 | 1 | CYT | Cp1002_0540 | Core |
| D9Q6C7_CORP1 | Hypothetical protein | 689.6 | 25 | 2.25 | 1 | PSE | Cp1002_0320 | Core |
| D9Q3P3_CORP1 | Hypothetical protein | 5703.38 | 3 | 1.87 | 1 | CYT | Cp1002_1474 | Core |
| D9Q5V4_CORP1 | Hypothetical protein | 994.52 | 1 | 1.7 | 1 | CYT | Cp1002_0143 | Core |
| D9Q610_CORP1 | Hypothetical protein | 27217.36 | 2 | 1.67 | 1 | CYT | Cp1002_0202 | Core |
| D9Q8D8_CORP1 | Hypothetical protein | 2324.12 | 7 | 1.57 | 0.98 | CYT | Cp1002_1048 | Shared |
| D9Q6W1_CORP1 | Hypothetical protein | 9303.91 | 4 | 1.54 | 1 | CYT | Cp1002_0512 | Core |
| D9Q6V5_CORP1 | Hypothetical protein | 1346.2 | 4 | 1.5 | 0.99 | CYT | Cp1002_0506 | Core |
| D9Q5R7_CORP1 | Hypothetical protein | 2090.7 | 8 | 1.42 | 1 | CYT | Cp1002_0105 | Core |
| D9Q917_CORP1 | Hypothetical protein | 555.89 | 10 | 1.37 | 1 | PSE | Cp1002_1281 | Core |
| D9Q3P5_CORP1 | Hypothetical protein | 1121.7 | 6 | 1.29 | 1 | SEC | Cp1002_1476 | Core |
| D9Q7U5_CORP1 | Hypothetical protein | 517.06 | 8 | 1.28 | 1 | CYT | Cp1002_0852 | Core |
| D9Q7L1_CORP1 | Hypothetical protein | 15693.97 | 6 | 1.28 | 1 | SEC | Cp1002_0766 | Core |
| D9Q3P6_CORP1 | Hypothetical protein | 1729.59 | 5 | 1.22 | 1 | CYT | Cp1002_1477 | Core |
| D9Q6Z7_CORP1 | Hypothetical protein | 1835.7 | 13 | 1.22 | 1 | CYT | Cp1002_0548 | Core |
| D9Q8V8_CORP1 | Hypothetical protein | 293.23 | 8 | -1.48 | 0 | Cp1002_1221 | Core | |
| D9Q6C8_CORP1 | Hypothetical protein | 413.31 | 12 | -1.52 | 0 | PSE | Cp1002_0321 | Core |
| D9Q5H0_CORP1 | Hypothetical protein | 12376.2 | 6 | -1.71 | 0 | CYT | Cp1002_0007 | Core |
| D9Q4D5_CORP1 | Hypothetical protein | 10161.64 | 4 | -4.29 | 0 | CYT | Cp1002_1721 | Shared |
| Others | ||||||||
| D9Q5N5_CORP1 | Iron-regulated MEM protein | 992.54 | 8 | 2.01 | 0 | PSE | piuB | Core |
| D9Q922_CORP1 | CobW/HypB/UreG, nucleotide-binding | 1771.22 | 20 | 1.88 | 1 | CYT | Cp1002_1286 | Core |
| D9Q8C4_CORP1 | Prokaryotic ubiquitin-like protein Pup | 2194.86 | 1 | 1.84 | 1 | CYT | pup | Core |
| D9Q7B8_CORP1 | Ribosomal-protein-alanine n-acetyltransferase | 2791.1 | 10 | 1.34 | 1 | CYT | rimJ | Shared |
| D9Q7K9_CORP1 | Arsenate reductase | 5147.54 | 8 | 1.32 | 1 | CYT | arsC | Core |
(a) Ratio values to: strain 1002_DETA/NO:strain 1002_Ct, Log(2) Ratio > 1.5, p > 0.95 = up-regulation, p < 0.05 = down-regulation.
(b) Core-genome analysis of 15 strains of C. pseudotuberculosis: shared = present in two or more strains; core = present in 15 strains of C. pseudotuberculosis.
(c) CYT =cytoplasmic, MEM = membrane, PSE = potentially surface-exposed, SEC = secreted.