Table 2. In vitro digestion of NMDA-R associated proteins by resident calpain-1 in isolated NR1 immunoprecipitates.

	Resident calpain-1 substrates
Conditions	HSP90	NR2B	160 kD nNOS	130 kD nNOS
Control	100 ± 6	100 ± 8	80 ± 6	22 ± 6
Vehicle	100 ± 8	100 ± 8	86 ± 5	18 ± 7
CaCl2 (1 mM)	80 ± 8	27 ± 8	32 ± 7	58 ± 8
CaCl2 (1mM) + CI-1 (1 μM)	100 ± 5	96 ± 8	79 ± 7	24 ± 6

SK-N-BE cells were lysed and the deoxycholate-soluble cell fraction was prepared, as described in Methods. The samples were submitted to immunoprecipitation using anti-NR1 antibody. The immunoprecipitated material was left untreated (Control) or incubated in the indicated conditions for 30 min at 37°C and then analyzed by immunoblotting for the listed proteins. The data shown are the quantification of the immunoreactive signals expressed as arbitrary units (means ± SD of five different experiments).