Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Hypertension. 2014 Dec 1;65(2):447–455. doi: 10.1161/HYPERTENSIONAHA.114.04179

FIGURE 1.

FIGURE 1

Phenotyping and embryo-transfer procedures. (A) Mean arterial pressure (MAP) and urinary albumin excretion (UAlb) were measured in SS/Mcw, SS/Crl and F1 offspring beginning at 7–8 weeks of age on C0.4 or G0.75 diets, respectively, for three days before switching animals to the C4.0 diet for 21 days. (B) An embryo transfer strategy to test the role of maternal in utero environmental exposure to the casein-based diet on blood pressure and renal injury. Embryos from SS/McwC0.4 mate pairs were transferred into either SS/McwC0.4 or SS/CrlG0.75 recipients (SS/McwC0.4>SS/McwC0.4, SS/McwC0.4>SS/CrlG0.75). Similarly, embryos from SS/CrlG0.75 mate pairs were transferred into those same recipients (SS/CrlG0.75>SS/McwC0.4, SS/CrlG0.75>SS/CrlG0.75).