Assessment of hypertensive phenotypes in embryo transferred F1 male rats. Following embryo transfer, offspring were phenotyped for (A) Mean arterial blood pressure (MAP) and (B) albumin excretion rate in response to being fed the C0.4 diet from weaning, followed by 21 days of the C4.0 diet. (C) Kidneys were extracted, sectioned, and trichrome stained to determine the level of renal damage between the groups. The SS/McwC0.4>SS/McwC0.4 and the SS/CrlG0.75>SS/McwC0.4 F1 kidneys were far more damaged compared to the SS/McwC0.4>SS/CrlG0.75 and the SS/CrlG0.75>SS/CrlG0.75 kidneys. (D) Consistent with these observations, SS/McwC0.4>SS/McwC0.4 and the SS/CrlG0.75>SS/McwC0.4 kidneys had significantly more severely-damaged glomeruli compared to the SS/McwC0.4>SS/CrlG0.75 and the SS/CrlG0.75>SS/CrlG0.75 glomeruli. *P<0.05 vs SS/McwC0.4>SS/CrlG0.75 and the SS/CrlG0.75>SS/CrlG0.75. Error bars represent SEM.