Figure 4.
Both forms of the heterodimeric IL-15 preferentially expand CD8+NK cells. (a) Expression of CD8 and Ki-67 within the CD3−CD16+ total NK cells subset is shown on day 0 and day 9 after the start of the treatment for the individual animals. (b) Blood samples were obtained at the indicated time points and stained with monoclonal antobodies binding to CD3, CD4, CD8 and CD16 and examined by flow cytometry. The data for CD3−CD16+ (Total NK cells, top panel), for CD3−CD16+CD8+ (CD8+NK cells, middle panel) and for CD3−CD16+CD8− (CD8−NK cells, bottom panel) are shown as absolute numbers per μl of blood on the indicated day relative to the absolute number per μl of blood of cells at the day of the start of the treatment. *P<0.05, in comparison to time point 0 (one-way analysis of variance test).