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. 2014 Dec 2;9:26. doi: 10.1186/1749-8104-9-26

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© Trivedi et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Myosin ii and F-actin dynamics motor activity are required for JAM-C and Cadm3 adhesion dynamics in migrating neurons. CGNs were transfected with expression vectors encoding JAM-C-pHluorin (A, B) or Cadm3-pHluorin (C, D). Time-lapse imaging was used to monitor cell surface adhesion dynamics in migrating cells. After cells were allowed to migrate for 18 to 50 minutes, 50 μM blebbistatin or 5 μM jasplakinolide were added to the culture and imaging continued for a further 28 minutes. Addition of either drug potently inhibited forward movement, JAM-C dynamics, and Cadm3 adhesions. Scale bar, 10 μm.