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. 2014 Nov 3;11:186. doi: 10.1186/1743-422X-11-186

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© Lovato et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of GALV-Nf in systemically-infected grapevine plants. (A) RT-PCR analysis of pK7WG2-MCS.HRz.GALV-Nf infected systemic leaves of (1) V. riparia, or V. vinifera cv. (2) Sultana, (3) Corvina, (4) Nebbiolo, (5) Syrah, (6) Brachetto, and on corresponding non-inoculated plants (7, 8, 9, 10, 11, 12). w: water negative control. Tissue-print immunoassay on systemically-infected Corvina leaf (B) and on a GALV-free leaf (C).