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. 2014 Nov 19;15(1):994. doi: 10.1186/1471-2164-15-994

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© Connelley et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Usage of TRAV/TRDV subgroups in the expressed TRA and TRD chain repertoires. cDNA from CD3⁺γδ^- (αβ T cells) and CD3⁺γδ⁺ (γδ T cells) populations isolated from the PBMC of 2 animals were subjected to unbiased SMART TRAC⁺/ TRDC⁺ PCR amplification and representative amplicons sequenced. The subgroup of the TRAV/TRDV gene expressed by each TRA and TRD chain sequence that was predicted from in silico analysis to be functional (for TRA transcripts n = 60 and 61 and for TRD transcripts n = 68 and 63 for Animals A and B respectively) was identified. The percentage of the TRA transcripts (A) and TRD transcripts (B) that utilised genes from the different TRAV/TRDV subgroups is shown (grey and black bars) alongside the percentage of the genes in the genomic repertoire available for TRA and TRD rearrangement within each TRAV/TRDV subgroup (striped bars).