Table 5.

Sequence of TRAV/TRDV subgroup-specific primers

TRAV/TRDV subgroup	Subgroup-specific 5′ primer sequence
AV1	CAGGAAAAGGCGTTAAGCAG
AV2	GGTCTCTTTGGAGGGAGCTG
AV3	CAGCCAGAAGCTGAGGTC
AV4	AGTGACCGTGCTCCTGAC
AV8.1	TGATGCTTGAGATGCTCCTG
AV8.2	CGGTCACATCAACGTCTCTG
AV8.3	ATTCCAGAGGCCAGTCAGTG
AV9	CTTCTCCAGGCTTAGTGACTG
AV10	TGAGTGGCAAAAACCAAGTG
AV12	CACAGTGGAGCAGAGTCCTG
AV5 and 13	CTTGTGGCT(A/G)CAGCTGGAC
AV14	AGGTGGTCGTGGCTTCACT
AV16	CAAGAGCCCAGACAGTGACTC
AV17	GAGAAGCTTCTGGCCCTG
AV18	GTTGTTACCCT(C/T)CCCGAGAAG
AV19	G(G/C)ATGTA(A/G)CCTTGAACTGTGC
AV20	CCAGGAGGGGGACAGTCT
AV21	GCCTGCTCATCCTTTGGTTA
AV22	CTT(C/T)TGTTTGCCCAGGTTTG
AV23	AAGTGACCAA(G/C)AGCAGGTGA
AV24	CCCTTGCTGTGGGTTCAG
AV25	G(G/C)ACCAGTGTTGATCTTA(C/T)GGA
AV26	TCCATGGATT(A/G)T(G/C)CTGAAGG
AV27	TGGTCTTTTGGATTCAACTGG
AV28	ACAAAGAAGAGTCTTGCTGAGTC
AV29	GCTTCAGTCTGACTGGGTG
AV33	GGCTGACAAAGTTACTGAAGC
AV35	GACATGTGTGAGTGCCCAAC
AV36	CCCCATCTCTGATTGTCCAT
AV38	CACAGTGACCCTGGACTGTA
AV41	AGGAAGGAGACCTCGTCACA
AVX	GGCTCTC(A/C)TGACCCTGAACT
AVY	GGTGGAGCAGA(A/G)TCCTTCAG
DV1.1	(G/C/T)C(A/T)GCTCTGGGTG(C/T)TCCT
DV1.2	GTGGCCCAGA(A/G)(A/C/T)GTTACTCA
DV2	TCATCCACCTCACCCTCTTC
DVY	CGTAACTGGAGGGAACTGGA
DV3	ATGTTTCTCCCTGTGGGCTTC
DVb3	TCTGCCTTGGTTCCAACAAT

All of these primers were designed as part of this study based on sequences of functional members of the TRAV/TRDV subgroups identified in the UMD3.1 genome assembly, with the exception of DV3 which was published previously [17].