Figure 4.

Expression profiles of Wolbachia metabolic pathways and genes of interest throughout the D . immitis life cycle. Heatmap showing the expression (normalized FPKM values) of genes involved in the synthesis of heme, riboflavin, purines, pyrimidines, folate, peptidoglycan/lipid II, isoprenoid, as well as components of the Sec translocase and the type IV secretion system. Each gene is represented by a single row. Data from biological replicates were combined prior to clustering. The color scale ranges from white (no expression) to black (very high expression).