Table 2.
ER, PR, HER2 and Gb3 expression in HBCx
| id | IHC | ESL | FACS | Gb3 extraction | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ER | PR | HER2 | Gb3 | n count | n pop | % min fluo | Ratio MFI | % max fluo | Ratio MFI | ng Gb3/mg of tissue | |
| BC11 | - | - | - | - | 31499 | 1 | 100 | 1.5 | x | x | N.A. |
| BC138 | - | - | - | + | 10579 | 2 | 85 | 1.2 | 15 | 13.1 | 2.7 |
| BC143 | - | - | - | - | 9704 | 1 | 100 | 1.1 | x | x | 0.0 |
| BC147 | - | - | - | + | 9051 | 2 | 85.6 | 2.9 | 14.7 | 22.9 | 45.7 |
| BC151 | - | - | + | - | 2032 | 1 | 100 | 1.0 | x | x | 0.0 |
| BC152 | - | - | - | - | 8444 | 1 | 100 | 1.0 | x | x | 15.0 |
| BC162 | - | - | - | + | 25850 | 1 | 100 | 5.8 | x | x | N.A. |
| BC174 | - | - | - | + | 26528 | 2 | 23.7 | 2.9 | 76.3 | 30.1 | 29.0 |
| BC389 | - | - | + | + | 27074 | 1 | 100 | 2.6 | x | x | N.A. |
| BC52 | + | - | - | + | 20355 | 2 | 67.4 | 3.8 | 32.6 | 29.7 | 200.0 |
| BC73 | - | - | - | - | 20583 | 1 | 100 | 1.2 | x | x | 5.9 |
+: positive; -: negative; n count: number of counted cells; n pop: number of present cell populations; % min fluo: percentage of cells expressing low or no Gb3 expression; % max fluo: percentage of cells with higher Gb3 expression. N.A.: data not available.