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. 2014 Nov 18;15(1):980. doi: 10.1186/1471-2164-15-980

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© Chen et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Disease progressions in N. benthamiana caused by P. cactorum isolate 10300. Leaves (A) or roots (B) were inoculated with a P. cactorum zoospore suspension. (A) Trypan blue-stained leaves at 3, 6, 12 and 24 h post-inoculation (hpi). On each leaf, the left side of the main vein was mock-inoculated with water while the right side was inoculated with zoospores. (B) Plants were inoculated by pouring zoospore suspension around the roots. Upper panel, disease development on whole plants 1, 2, 3, 4, 5 days post-inoculation (dpi). Lower panel, root from plants in upper panel experiments were cut open to observe symptoms inside the roots. Ctrl, roots from control plants mock-inoculated with water.